Rat Glycated Haemoglobin (GHb) ELISA Kit

Cat.#:

JL21291

Regulatory Status:

RUO

Alternative:

Glycated haemoglobin

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.25-2000ng/mL

Sensitivity:

15.3ng/mL

Specificity:

This kit recognizes Rat GHb in samples. No significant cross-reactivity or interference between Rat GHb and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Metabolism

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| GHb

Glycated hemoglobin, also called glycohemoglobin, is a form of hemoglobin (Hb) that is chemically linked to a sugar.Most monosaccharides, including glucose, galactose, and fructose, spontaneously (that is, non-enzymatically) bond with hemoglobin when they are present in the bloodstream. Glycated hemoglobin causes an increase of highly reactive free radicals inside blood cells, altering the properties of their cell membranes. This leads to blood cell aggregation and increased blood viscosity, which results in impaired blood flow.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat GHb spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-102	96
Plasma(n=8)	92-106	103
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Rat GHb and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-96	88-97	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-104	87-108	105-114
1 : 4	Average (%)	94	98	107