| GApo-A

Apolipoprotein A (APO-A)‌ is the primary structural protein of high-density lipoprotein (HDL) and plays a critical role in lipid metabolism and cardiovascular health. APO-A facilitates the clearance of cholesterol deposits from blood vessel walls by binding cholesterol and promoting its reverse transport to the liver for metabolism. The APO-A family includes subtypes such as A1, A2, A4, and A5, among which ‌Apo A1‌ is the most significant, constituting over 70% of HDL's total protein content. Apo A1 activates the ‌lecithin-cholesterol acyltransferase (LCAT)‌ enzyme to drive reverse cholesterol transport, shuttling cholesterol from peripheral tissues to the liver for metabolism, thereby counteracting atherosclerosis.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	2000	1000	500	250	125	62.5	31.25	0
OD	2.02	1.22	0.75	0.47	0.3	0.22	0.18	0.08
Corrected OD	1.94	1.14	0.67	0.39	0.22	0.14	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat GApo-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-102	95
Plasma(n=8)	92-106	103
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Rat GApo-A and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-95	89-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-114
	Average (%)	94	98	107