| HMGB-1

High mobility group protein 1(HMGB-1), also known as high-mobility group protein 1 (HMG-1) and amphoterin.HMG-1 belongs to the high mobility group and contains a HMG-box domain.Like the histones, HMGB1 is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones.This nuclear protein organizes the DNA and regulates transcription.After binding, HMGB1 bends DNA, which facilitates the binding of other proteins. HMGB1 supports transcription of many genes in interactions with many transcription factors. It also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. Contact with core histones changes the structure of nucleosomes.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat HMGB-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	90-105	102

 

| Linearity

Samples were spiked with high concentrations of Rat HMGB-1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-96	87-98
	Average (%)	91	93
1 : 4	Range (%)	89-103	86-108
	Average (%)	94	98