| Ser536

Phosphorylated NF-κB p65 (Ser536) represents a critical regulatory site within the NF-κB signaling pathway. NF-κB is an essential transcription factor that participates in a wide array of physiological and pathological processes, including inflammation, immunity, cell proliferation, and apoptosis. The phosphorylation of the Ser536 site on the NF-κB p65 subunit enhances its transcriptional activity, thereby promoting the expression of downstream target genes. This phosphorylation event is typically mediated by the IKK kinase complex or other kinases (such as TBK1, MSK1) in response to stimulation by pro-inflammatory factors like TNF-α and IL-1β, or pathogen-associated molecular patterns (PAMPs).
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.64	1.92	1.19	0.73	0.45	0.29	0.21	0.08
Corrected OD	2.56	1.84	1.11	0.65	0.37	0.21	0.13	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse Ser536 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-110	105

 

| Linearity

Samples were spiked with high concentrations of Mouse Ser536 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-93	88-96	90-110
	Average (%)	91	93	97
1 : 4	Range (%)	89-103	87-110	105-115
	Average (%)	94	98	109