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Mouse Low Density LipoProtein Cholesterol (LDL-C) ELISA Kit

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Product Name:

Mouse Low Density LipoProtein Cholesterol (LDL-C) ELISA Kit

Cat.#:

JL20313

Regulatory Status:
Alternative:

low density lipoprotein cholesterol

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
3.12-200ng/mL
Sensitivity:
1.56ng/mL
Specificity:
This kit recognizes Mouse LDL-C in samples. No significant cross-reactivity or interference between Mouse LDL-C and analogues was observed.
Sample Type:
Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids
Assay Procedure:
Assay Procedure
Research Area:
Cardiovascular Biology
  • Mouse Low Density LipoProtein Cholesterol (LDL-C) ELISA Kit
  • Mouse Low Density LipoProtein Cholesterol (LDL-C) ELISA Kit
  • Mouse Low Density LipoProtein Cholesterol (LDL-C) ELISA Kit
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Product Datasheets
Instruction: Down Instruction


| LDL-C

Very low-density lipoprotein cholesterol (VLDL-C) is primarily synthesized in the liver and plays a crucial role in transporting endogenously produced triglycerides (TGs). Its degradation is influenced by various factors, including diet, enterhepatic circulation, capillary endothelial function, and hormones such as adrenaline. VLDL (very low-density lipoprotein) is a lipoprotein particle produced in the liver, carrying triglycerides and cholesterol. VLDL-C specifically refers to the cholesterol content within VLDL particles, serving as an important biomarker for lipid metabolism assessment.



| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Mouse LDL-C spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 
Sample Type Range(%) Average Recovery(%)
Serum(n=8) 84-101 96
Plasma(n=8) 92-105 102
Cell Culture Supernates(n=8) 96-107 104





 

| Linearity

Samples were spiked with high concentrations of Mouse LDL-C and diluted with Universal Diluent to produce samples with values within the range of the assay.

 
    Serum(n=4) Plasma(n=4) Cell Culture Supernates(n=4)
1 : 2 Range (%) 84-95 87-96 90-110
Average (%) 91 92 96
1 : 4 Range (%) 89-103 87-108 105-115
Average (%) 94 98 108







 
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Sample Data
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Sample Data
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Sample Data
Size Quantity Price ($)
48T
1
360 / 360
96T
1
460 / 460
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