| PHF8

PHD finger protein 8 is a protein that is encoded by the PHF8 gene.PHF8 belongs to the family of ferrous iron and alpha-ketoglutarate-dependent hydroxylases superfamily, and is active as a histone lysine demethylase with selectivity for the di-and monomethyl states. PHF8 induces an EMT (epithelial to mesenchymal transition)-like process by upregulating key EMT transcription factors SNAI1 and ZEB1.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.47	1.91	1.34	0.87	0.61	0.42	0.31	0.09
Corrected OD	2.38	1.82	1.25	0.78	0.52	0.33	0.22	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse PHF8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-100	96

 

| Linearity

Samples were spiked with high concentrations of Mouse PHF8 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95
	Average (%)	90
1 : 4	Range (%)	89-102
	Average (%)	94