Home > Sandwich ELISA Kits > Mouse suppressors of cytokine signaling 1 (SOCS-1) ELISA Kit

Product Name:

Mouse suppressors of cytokine signaling 1 (SOCS-1) ELISA Kit

Cat.#:

JL53966

Regulatory Status:

RUO

Alternative:

CIS1; CISH1; JAB; SSI-1; SSI1; TIP3; JAK-binding protein; STAT-induced STAT inhibitor 1; Tec-interacting protein 3

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

62.5-4000pg/mL

Sensitivity:

31.4pg/mL

Specificity:

This kit recognizes Mouse SOCS-1 in samples. No significant cross-reactivity or interference between Mouse SOCS-1 and analogues was observed.

Sample Type:

Tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| SOCS-1

Suppressors of cytokine signaling 1(SOCS-1) is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signalling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this protein can be induced by a subset of cytokines, including IL2, IL3, erythropoietin (EPO), GM-CSF, and interferon-gamma (IFN-γ). The protein functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-γ action, which is required for normal postnatal growth and survival.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse SOCS-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Mouse SOCS-1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	90-110
1 : 2	Average (%)	91	96
1 : 4	Range (%)	89-103	105-114
1 : 4	Average (%)	94	108