Home > Sandwich ELISA Kits > Monkey Solubility Cluster Of Differentiation 40 Ligand (sCD40L) ELISA Kit

Product Name:

Monkey Solubility Cluster Of Differentiation 40 Ligand (sCD40L) ELISA Kit

Cat.#:

JL54658

Regulatory Status:

RUO

Alternative:

Cd40l ; Tnfsf5;CD40 ligand ; CD40-L ; T-cell antigen Gp39 ; TNF-related activation protein ; TRAP ; Tumor necrosis factor ligand superfamily member 5 ; CD antigen CD154;Cleaved into: CD40 ligand ; membrane form; CD40 ligand ; soluble form ; sCD40L

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.068ng/mL

Specificity:

This kit recognizes Monkey sCD40L in samples. No significant cross-reactivity or interference between Monkey sCD40L and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| sCD40L

The soluble form of CD40 ligand (sCD40L) has garnered increasing attention as a potential biomarker in cancer diagnosis and progression. Shed from the cell surface upon activation, sCD40L enters circulation and reflects the dynamic interplay between immune cells and the tumor microenvironment. Emerging evidence suggests that alterations in sCD40L levels may correlate with cancer development, aggressiveness, and treatment response, thereby holding promise as a non-invasive tool for cancer detection and monitoring.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Monkey sCD40L spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	94-105	103
Cell Culture Supernates(n=8)	96-108	106

| Linearity

Samples were spiked with high concentrations of Monkey sCD40L and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-95	88-97	90-111
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-102	87-109	105-114
1 : 4	Average (%)	95	98	106