Home > Sandwich ELISA Kits > Human Soluble Vascular Endothelial Growth Factor Receptor-1 (sVEGFR-1) ELISA Kit

Product Name:

Human Soluble Vascular Endothelial Growth Factor Receptor-1 (sVEGFR-1) ELISA Kit

Cat.#:

JL19608

Regulatory Status:

RUO

Alternative:

Soluble vascular endothelial growth factor receptor 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78.1-5000pg/mL

Sensitivity:

40pg/mL

Specificity:

This kit recognizes Human sVEGFR-1 in samples. No significant cross-reactivity or interference between Human sVEGFR-1 and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;saliva;cell lysates;follicular fluid and other biological fluids

Assay Procedure:

Research Area:

Cell Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| sVEGFR-1

Soluble vascular endothelial growth factor receptor-1 (sVEGFR-1, also known as sFlt-1) is a type of vascular endothelial growth factor receptor (VEGFR) competitor. The VEGFR1 gene is located on chromosome 13q126,7. It was initially found to be expressed in vascular endothelial cells 8, and then found to be expressed in smooth muscle cells 9, monocytes 10, trophoblast cells 11, interstitial cells 12 and osteoblasts 13. After combining with vascular endothelial growth factor, it has a negative effect on angiogenesis. However, sVEGFR-1 interacts with endothelial cell components, showing an angiogenic effect. Therefore, sVEGFR-1 has multiple effects on the progression of vascular growth and may be a new therapeutic target for VEGF-mediated pathological signals.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	5000	2500	1250	625	312.5	156.2	78.1	0
OD	2.66	2.04	1.51	1.04	0.65	0.39	0.22	0.09
Corrected OD	2.57	1.95	1.42	0.95	0.56	0.3	0.13	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human sVEGFR-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human sVEGFR-1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-107	105-115
1 : 4	Average (%)	94	98	108