Home > Sandwich ELISA Kits > Human Solute Carrier Family 39, Member 4 (SLC39A4) ELISA Kit

Product Name:

Human Solute Carrier Family 39, Member 4 (SLC39A4) ELISA Kit

Cat.#:

JL16883

Regulatory Status:

RUO

Alternative:

ZIP4; AEZ; Acrodermatitis Enteropathica;Zinc-Deficiency Type; Zinc Transporter Protein 4; Solute Carrier Family 39; Member 4; Zrt-and Irt-like protein 4

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.077ng/mL

Specificity:

This kit recognizes Human SLC39A4 in samples. No significant cross-reactivity or interference between Human SLC39A4 and analogues was observed.

Sample Type:

tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| SLC39A4

Zinc transporter ZIP4 is a transmembrane protein which is encoded by the SLC39A4 gene. It is associated with acrodermatitis enteropathica.This gene encodes a member of the zinc/iron-regulated transporter-like protein (ZIP) family. The encoded protein localizes to cell membranes and is required for zinc uptake in the intestine. Mutations in this gene result in acrodermatitis enteropathica. Multiple transcript variants encoding different isoforms have been found for this gene.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.14	1.63	1.16	0.78	0.57	0.36	0.24	0.07
Corrected OD	2.07	1.56	1.09	0.71	0.5	0.29	0.17	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human SLC39A4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-100	96
Cell Culture Supernates(n=8)	92-105	103

| Linearity

Samples were spiked with high concentrations of Human SLC39A4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	90-103	87-108
1 : 4	Average (%)	94	99