Product Name:
Human Thioredoxin-related Transmembrane Protein 2 (TMX2) ELISA Kit
Cat.#:
JL52543
Regulatory Status:
Alternative:
TMX2
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.073ng/mL
Specificity:
This kit recognizes Human TMX2 in samples. No significant cross-reactivity or interference between Human TMX2 and analogues was observed.
Sample Type:
Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids
Assay Procedure:
Research Area:
Metabolism
Size & Price: 
Product Datasheets
Instruction: 
| TMX2
Thioredoxin Related Transmembrane Protein 2(TMX2) a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions.It has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, one transmembrane domain and a C-terminal ER-retention sequence. This protein is enriched on the mitochondria-associated-membrane of the ER via palmitoylation of two of its cytosolically exposed cysteines.
| Repeatability
Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%| Recovery
The recovery of Human TMX2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.| Sample Type | Range(%) | Average Recovery(%) |
| Serum(n=8) | 84-101 | 96 |
| Plasma(n=8) | 93-105 | 103 |
| Cell Culture Supernates(n=8) | 96-108 | 105 |
| Linearity
Samples were spiked with high concentrations of Human TMX2 and diluted with Universal Diluent to produce samples with values within the range of the assay.| Serum(n=4) | Plasma(n=4) | Cell Culture Supernates(n=4) | ||
| 1 : 2 | Range (%) | 84-95 | 88-96 | 90-110 |
| Average (%) | 91 | 93 | 95 | |
| 1 : 4 | Range (%) | 89-103 | 87-108 | 105-113 |
| Average (%) | 94 | 98 | 107 |
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