| P-ULK1

Serine/threonine-protein kinase ULK1 is an enzyme that is encoded by the ULK1 gene. Unc-51-like autophagy-activating kinases 1 and 2 (ULK1/2) are two similar isoforms of an enzyme that in humans is encoded by the ULK1/2 genes. The enzyme is specifically a kinase that is involved with autophagy, particularly in response to amino acid withdrawal. Not many studies have been done comparing the two isoforms, but some differences have been recorded.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.36	1.7	1.26	0.87	0.6	0.4	0.28	0.09
Corrected OD	2.27	1.61	1.17	0.78	0.51	0.31	0.19	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human P-ULK1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	92-106	102

 

| Linearity

Samples were spiked with high concentrations of Human P-ULK1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	88-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	98