| Cyclin-D1

Cyclin-D1 (Cyclin-D1) is a protein encoded by the CCND1 gene. The CCND1 gene encodes the cyclin D1 protein, which is located on the long arm of chromosome 11 (band 11q13). The gene spans 13,388 base pairs and is translated into 295 amino acids. Except for cells derived from bone marrow stem cell lineages (including lymphoid and myeloid lineages), cyclin D1 is expressed in all adult tissues. Its overexpression has been shown to be associated with early cancer onset and tumor progression. It can contribute to tumorigenesis by enhancing anchorage-dependent growth and angiogenesis through increased VEGF production.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.29	1.64	1.08	0.7	0.44	0.28	0.19	0.08
Corrected OD	2.21	1.56	1	0.62	0.36	0.2	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human Cyclin-D1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-107	105

 

| Linearity

Samples were spiked with high concentrations of Human Cyclin-D1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	87-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-114
	Average (%)	94	98	107