Human Mast Cell-like Trypsin (MCT) ELISA Kit

Cat.#:

JL12975

Regulatory Status:

RUO

Alternative:

MCT;Tryptase alpha/beta-1;Tryptase-1;Tryptase I;Tryptase alpha-1;TPSAB1;TPS1;TPS2;TPSB1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.76ng/mL

Specificity:

This kit recognizes Human MCT in samples. No significant cross-reactivity or interference between Human MCT and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MCT

Mast cell-like tryptase namely Tryptase is the most abundant secretory granule-derived serine proteinase contained in mast cells and has been used as a marker for mast cell activation.Club cells contain tryptase, which is believed to be responsible for cleaving the hemagglutinin surface protein of influenza A virus, thereby activating it and causing the symptoms of flu.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	100	50	25	12.5	6.25	3.12	1.56	0
OD	2.56	1.45	0.8	0.48	0.33	0.23	0.2	0.08
Corrected OD	2.48	1.37	0.72	0.4	0.25	0.15	0.12	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human MCT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	85-101	96
Plasma(n=8)	92-106	103
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human MCT and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	88-107	105-114
1 : 4	Average (%)	94	97	107