Home > Sandwich ELISA Kits > Human Pulmonary Surfactant-associated Protein C (SP-C) ELISA Kit

Product Name:

Human Pulmonary Surfactant-associated Protein C (SP-C) ELISA Kit

Cat.#:

JL11024

Regulatory Status:

RUO

Alternative:

BRICD6; BRICHOS domain containing 6; PSP C; PSPC; PSPC_HUMAN; Pulmonary surfactant apoprotein 2; Pulmonary surfactant apoprotein PSP C; pulmonary surfactant apoprotein-2 SP-C; Pulmonary surfactant associated protein C; Pulmonary surfactant associated prot

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.153ng/mL

Specificity:

This kit recognizes Human SP-C in samples. No significant cross-reactivity or interference between Human SP-C and analogues was observed.

Sample Type:

Serum; plasma; tissue homogenates and other biological fluids

Assay Procedure:

Research Area:

Epigenetics

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| SP-C

Surfactant protein C (SP-C), is one of the pulmonary surfactant proteins. It is encoded by the SFTPC gene.It is a membrane protein.SFTPC is a 197-residue protein made up of two halves: a unique N-terminal propeptide domain and a C-terminal BRICHOS domain. The around 100-aa long propeptide domain actually contains not only the cleaved part, but also the mature peptide. It can be further broken down into a 23-aa helical transmembrane propeptide proper, the mature secreted SP-C (24-58), and a linker (59-89) that connects to the BRICHOS domain.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.22	1.56	0.92	0.63	0.42	0.25	0.18	0.08
Corrected OD	2.14	1.48	0.84	0.55	0.34	0.17	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human SP-C spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	95-108	104

| Linearity

Samples were spiked with high concentrations of Human SP-C and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-107	105-115
1 : 4	Average (%)	94	97	108