| ATP1b4

ATP1B4 (ATPase Na+/K+ Transporting Family Member Beta 4) is a Protein Coding gene. Diseases associated with ATP1B4 include Autism Spectrum Disorder and Mannosidosis, Beta A, Lysosomal.An important paralog of this gene is ATP1B2.ATPases are a class of enzymes that catalyze the decomposition of ATP into ADP and a free phosphate ion or the inverse reaction. This dephosphorylation reaction releases energy, which the enzyme (in most cases) harnesses to drive other chemical reactions that would not otherwise occur. This process is widely used in all known forms of life.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.07	1.48	0.94	0.65	0.43	0.31	0.24	0.08
Corrected OD	1.99	1.4	0.86	0.57	0.35	0.23	0.16	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human ATP1b4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	96-106	99

 

| Linearity

Samples were spiked with high concentrations of Human ATP1b4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)
1 : 2	Range (%)	92-110
	Average (%)	96
1 : 4	Range (%)	105-115
	Average (%)	110