| aZGP1

Zinc-alpha-2-glycoprotein is a protein that in humans is encoded by the AZGP1 gene.AZGP1, also referred to as zinc-alpha-2-glycoprotein (ZAG), is a glycoprotein with a molecular weight of 38-40 kDa.AZGP1 is widely expressed in various tissues and body fluids, including the breast, stomach, liver, prostate, plasma, urine, and saliva.This gene expresses a soluble protein that stimulates lipolysis, induces a reduction in body fat in mice, is associated with the cachexia related to cancer, and is known to be expressed in secretory cells of lung epithelium.In 2009, it was found that smoking increases expression of this gene, which is why smoking cessation leads to weight gain.Decreased circulating AZGP1 levels are associated with diabetes 2.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	80	40	20	10	5	2.5	1.25	0
OD	2.36	1.78	1.23	0.79	0.46	0.29	0.17	0.09
Corrected OD	2.27	1.69	1.14	0.7	0.37	0.2	0.08	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human aZGP1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Human aZGP1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-99
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	97	98