| SKIL

Ski-like protein is a protein that is encoded by the SKIL gene.SKIL interacts with SKI protein, Mothers against decapentaplegic homolog 3 and Mothers against decapentaplegic homolog 2.SKIL belongs to the Ski/Sno/Dac family, shared by SKI protein, Dachshund, and SKIDA1. Members of the Ski/Sno/Dac family share a domain that is roughly 100 amino acids long.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	2000	1000	500	250	125	62.5	31.25	0
OD	2.56	1.77	1.22	0.71	0.46	0.31	0.23	0.07
Corrected OD	2.49	1.7	1.15	0.64	0.39	0.24	0.16	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human SKIL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	102
Tissue Homogenates(n=8)	96-108	104

 

| Linearity

Samples were spiked with high concentrations of Human SKIL and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95	85-96	89-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	93	98	108