| IGF1

Insulin-like growth factor 1 (IGF-1), also known as growth hormone C, is a hormone with a molecular structure similar to insulin. IGF-1 binds to at least two cell surface receptor tyrosine kinases: the IGF-1 receptor (IGF1R), and the insulin receptor. Its primary action is mediated by binding to its specific receptor, IGF1R, which is present on the surface of many cell types in many tissues. IGF-1 is one of the most potent natural activators of the Akt signaling pathway, a stimulator of cell growth and proliferation, and a potent inhibitor of programmed cell death.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	5000	2500	1250	625	312.5	156.25	78.12	0
OD	2.51	1.96	1.24	0.72	0.46	0.3	0.22	0.09
Corrected OD	2.42	1.87	1.15	0.63	0.37	0.21	0.13	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Dog IGF1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	88-103	99
Plasma(n=8)	94-107	104
Cell Culture Supernates(n=8)	96-110	107

 

| Linearity

Samples were spiked with high concentrations of Dog IGF1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-93	88-97	90-110
	Average (%)	91	93	97
1 : 4	Range (%)	89-103	87-110	105-115
	Average (%)	94	98	109